Cell-to-cell heterogeneity of phosphate gene expression in yeast is controlled by alternative transcription, 14-3-3 and Spl2

Dependent on phosphate availability the yeast Saccharomyces cerevisiae expresses either low or high affinity transporters. In presence of cells still express levels transporter Pho84. The regulator Spl2 is expressed in approximately 90% cells, and not remaining cells. Here we report that deletion RRP6, encoding an exonuclease degrading non-coding RNA, BMH1, major 14-3-3 isoform, resulted less expressing SPL2 increased RNA transcribed from sequences upstream coding region. stimulates its own expression PHO84 ensuing a positive feedback. Upon region responsible for transcription almost all SPL2. These results indicate cell-to-cell variation dependent specific part promoter controlled by Bmh1 Spl2.